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| Registros recuperados: 89 | |
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| Tu,Zhiming; He,Guangyuan; Li,Kexiu X.; Chen,Mingjie J.; Chang,Junli; Chen,Ling; Yao,Qing; Liu,Dongping P.; Ye,Huan; Shi,Jiantao; Wu,Xuqian . |
| This paper describes an efficient bacterial transformation system that was established for the preparation of competent cells, plasmid preparation, and for the storage in bacterial stocks in our laboratory. Using this method, a number of different plasmids have been amplified for further experiments. Competent cells for bacterial transformation were prepared by the calcium chloride method with an optimum concentration of 75 mM. Three different strains of Escherichia coli that were tested are DH5α, TG1 and XL1 blue, and the most efficient strain being XL1 blue. The optimal optical density (OD600) range for competent cell preparation varied for each of the strains investigated, and for XL1 blue it was 0.15-0.45; for TG1 it was 0.2-0.5; and for... |
| Tipo: Journal article |
Palavras-chave: Competent cells; E. coli; Plasmid; Storage; Transformation. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100014 |
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| Menezes,Caroline Anunciação; Gonçalves,Danielle Silva; Amianti,Jackeline; Fernandes,Irene; Taddei,Carla Romano; Koga,Paula Célia Mariko; Trabulsi,Luiz Rachid; Martinez,Marina Baquerizo; Piazza,Roxane Maria Fontes. |
| A capture enzyme-linked immunosorbent assay (ELISA), which detects LT-I toxin produced by enterotoxigenic Escherichia coli strains, has beendeveloped. This capture assay was performed using the IgG enriched fraction of anti-LT-I antiserum and IgG2b anti-LT-I monoclonal antibody and allowed a clear distinction between E. coli LT-I - producing and non-producing strains. The estimated accuracy of the assay is 78% for sensitivity, 94% for specificity and 92% for efficiency. Thus, the capture immunoassayis a sensitive tool for detection of E. coli, which produces heat-labile enterotoxin, and is suitable for use in clinical laboratories and epidemiological surveys in developing world. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Detection; Polyclonal antibody; Monoclonal antibody; Thermo-labile toxin; E. coli. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500004 |
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| Stoppe,Nancy de Castro; Silva,Juliana Saragiotto; Torres,Tatiana Teixeira; Carlos,Camila; Hachich,Elayse Maria; Sato,Maria Inês Zanoli; Saraiva,Antonio Mauro; Ottoboni,Laura Maria Mariscal. |
| Different types of water bodies, including lakes, streams, and coastal marine waters, are often susceptible to fecal contamination from a range of point and nonpoint sources, and have been evaluated using fecal indicator microorganisms. The most commonly used fecal indicator is Escherichia coli, but traditional cultivation methods do not allow discrimination of the source of pollution. The use of triplex PCR offers an approach that is fast and inexpensive, and here enabled the identification of phylogroups. The phylogenetic distribution of E. coli subgroups isolated from water samples revealed higher frequencies of subgroups A1 and B2(3) in rivers impacted by human pollution sources, while subgroups D1 and D2 were associated with pristine sites, and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: E. coli; Phylogenetic groups; Pollution sources; Interaction networks; Social network analysis. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000500012 |
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| Comlekcioglu,Ugur; Gunes,Merva; Altun,Hanifi; Ekiz,Dilek Ozgun; Aygan,Ashabil. |
| ABSTRACT Rumen fungi inhabit the gastro-intestinal tract of ruminants and the most non-ruminant herbivores. Rumen fungi produce highly active plant cell wall degrading enzymes, therefore they have gained scientific interest. In this study, genes encoding xylanase (xynA-7) and cellulase (celA-5) were amplified from Neocallimastix sp. GMLF7 and Orpinomyces sp. GMLF5, respectively, and expressed in Escherichia coli. XynA-7 was found to be active only on xylan, however CelA-5 had activity both on carboxymethyl cellulose and lichenan. Lichenase activity of CelA-5 was found to be higher than carboxymethyl cellulase activity. The optimal conditions were at pH 6.0 and 40 °C for CelA-5 and at pH 6.5 and 50 °C for XynA-7. A coexpression vector was constructed to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rumen fungi; Xylanase; Cellulase; Coexpression; E. coli. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100407 |
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| Martinez,Pedro; Garzón,Denisse; Mattar,Salim. |
| OBJECTIVE: Describe the presence of CTX-M-1 phylogenetic subgroup extended-spectrum i-lactamases (ESBL), associated with TEM and SHV genes, and the gene encoding cephalosporinase, CMY-2 in Escherichia coli and Klebsiella pneumoniae isolates from community-acquired urinary tract infections. METHODS: 102 E. coli and 21 K. pneumoniae were collected from patients with culture-proven urinary tract infection (UTI), during February and March, 2011. Antimicrobial susceptibility test was performed by disk diffusion according to the standards of the Clinical Laboratory Standard Institute. Screening for cephalosporins-resistant E. coli and K. pneumoniae was performed by PCR assay for blaTEM, blaSHV, blaCTX-M-1,-2,-8,-9, blaPER-2 and blaCMY-2 genes. Statistical... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ESBL; CTX-M; E. coli; K. pneumonia; Community-acquired UTIs; Colombia. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000500003 |
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| Sá,M.F.; Aita,C.; Doneda,A.; Pujol,S.B.; Cantú,R.R.; Jacques,I.V.C.; Bastiani,G.G.; Oliveira,P.D.; Lopes,P.D.. |
| Microrganismos presentes em dejetos de suínos podem contaminar o meio ambiente. Embora a compostagem seja preconizada como um método eficiente para reduzir este potencial poluidor dos dejetos, existem poucas informações de pesquisa sobre tal processo. O presente trabalho teve o objetivo de avaliar a eficiência da compostagem automatizada dos dejetos líquidos de suínos (DLS) na redução da população de coliformes, usados como indicadores de poluição fecal. Os DLS foram adicionados periodicamente, durante 106 dias, em substrato constituído pela mistura, em partes iguais, de maravalha e serragem. Foram efetuadas 14 adições de DLS, e em cada adição as leiras de compostagem eram revolvidas por meio de uma máquina especialmente desenvolvida para este fim. Foram... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: E. coli; Resíduos da suinocultura; Tratamento de efluentes. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000401197 |
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| Juang,J; Yin,H; Zhang,C; Wang,J. |
| ABSTRACT This experiment aimed to investigate whether Escherichia coli (E. coli) infection could affect the TGF-β/smads signaling pathway in the jejunal tissue of chickens. One-day-old Cobb 500 broilers were randomly divided into 2 groups and treated with intraperitoneal E. coli or broth injection. Clinical signs of the birds were assessed every day. Spleen and bursa of Fabricius of the birds, post-infection (pi), were collected to evaluate immune organ index. Jejunal tissues were collected to ascertain the expression of TGF-βs, TβRs, and Smads. The results showed that the infected birds had significantly higher index of the spleen (24hrs and 48hrs pi) compared with birds in the control group (p<0.05). The relative gene expression of TGF-β4 increased... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: TGF-β/Smads; Intestinal lesion; E. coli; Chicken. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000100311 |
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| Serais, Ophelie; Derolez, Valerie; Caprais, Marie-paule. |
| Microbes regularly contaminate Thau lagoon shellfish. Between 1997 and 2007, 18% of the analyses carried out in dry weather recorded more than 230 Escherichia coli / 100 g of bivalves (Ifremer French microbial monitoring network), leading the administration to restrict the shellfish harvest area, including suspension of production. This study aims to determine, the contribution of seabirds, which roost at night on the Thau lagoon shellfish growing frames, to shellfish faecal pollution in dry weather. Data were collected in 2008 from both the drainage area (pollution input) and the lagoon (water quality and microbial monitoring, seabird counts) in dry weather. The data showed that, with no watershed input or lagoon resurgence, microbial contaminants were... |
| Tipo: Text |
Palavras-chave: Etang de Thau; Contamination microbiologique; Contamination aviaire; E. coli; Coquillages. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00042/15337/12689.pdf |
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| Marzin, Anahita; Crottier, Anais; Piquet, Jean-come; Abadie, Eric; Roque D'Orbcastel, Emmanuelle; Fiandrino, Annie. |
| L’étude de zone des lotissements conchylicoles de la lagune de Thau (34.39) a été menée, à la demande du Comité Régional de la Conchyliculture en Méditerranée et du préfet de l’Hérault, pour étudier la possibilité de sectoriser la zone de production en sous-zones, au regard du risque microbiologiques (REMI). Une étude de dossier a été réalisée afin de recenser les principales sources de contamination. Celles-ci sont essentiellement d’origine anthropique et impactent différemment les secteurs envisagés. Des prélèvements de coquillages du groupe 3 ont été réalisés de mai 2017 à mai 2018 avec une fréquence bimensuelle. Au regard des critères du règlement (CE) n° 854/2004, les résultats des analyses microbiologiques (indicateur Escherichia coli) ont permis... |
| Tipo: Text |
Palavras-chave: REMI; E. coli; Contamination bactériologique des coquillages; ROCCH; Contaminants chimiques; Milieu marin; Étude de zone; Lagune de Thau; Département de l’Hérault. |
| Ano: 2018 |
URL: http://archimer.ifremer.fr/doc/00459/57056/58965.pdf |
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| Morin, Dimitri. |
| Après un rappel des objectifs, du fonctionnement et de la méthode d’interprétation des résultats du réseau de contrôle microbiologique REMI, ce rapport présente le bilan national et décrit le programme annuel pour le département de la Charente-Maritime. Il présente l’ensemble des résultats obtenus, en particulier l’estimation de la qualité des zones de production de coquillages classées et l’évolution de leur qualité sur la période 2014-2016. Le programme de surveillance 2016 a été réalisé à 99%. Les données manquantes résultent d’un manque de ressource ou de problèmes de disponibilité. Au total, le dispositif d’alerte a été déclenché à 25 reprises en 2016 et a donné lieu à la réalisation de 37 prélèvements et analyses supplémentaires. Toutes les zones de... |
| Tipo: Text |
Palavras-chave: Ifremer; REMI; E. coli; Contamination bactériologique des coquillages; Classement sanitaire des zones de production; Charente-Maritime.. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00384/49509/50003.pdf |
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| Registros recuperados: 89 | |
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